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rabbit anti pdgfr β antibody  (R&D Systems)


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    R&D Systems rabbit anti pdgfr β antibody
    Rabbit Anti Pdgfr β Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pdgfr+beta/Human%2FMouse+Phospho-PDGF+R+beta+(Y751)+Antibody/bio_rxiv__2025__10__13__681886-47-14-19
    Average 93 stars, based on 5 article reviews
    rabbit anti pdgfr β antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..

    Immunofluorescence:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..

    Staining:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..

    Labeling:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..

    Generated:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..

    RNA Sequencing:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..

    Negative Control:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..

    Construct:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..

    Control:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..

    Expressing:

    Article Title: A bioprinted and scalable model of human tubulo-interstitial kidney fibrosis.
    Article Snippet: The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).The sections were counterstained with DAPI (4′,6′-′diamidino-2-phenylindole) (Roche, 1:1000), washed in PBS and mounted with Immu-Mount (Thermo scientific, # 9990402).. The following primary antibodies were used: human PDGFR beta (R&D System, MAB1263,1:100), human CD10 (Biolegend, 312214, 1:100), mouse-CD31(R&D System, AF3628, 1:100), human Fibronectin (Abcam, ab23750, 1:100), CD13 (Thermo Fisher, 11-0138-42, 1:100), POSTN(Sigma, HPA012306, 1:100), aSMA (R&D systems, MAB1420, 1:100), Col1a1(Biozol, 1310-01, 1:100), Cubilin (Sigma, HPA004133, 1:100), vWF (Sigma, HPA001815, 1:100), AF405 donkey anti-mouse (Dianova, 715-475-150, 1:200).. The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit (Dianova, 711-605-152, 1:100), Cy3 donkey anti-mouse (Dianova, 715-165-151, 1:100), AF405 donkey anti-goat (Dianova, 705-475-147, 1:200).The following secondary antibodies were used: AF488 donkey anti-goat (Dianova, 705-545-147, 1:100), AF488 donkey anti-mouse (Dianova, 715-545-151, 1:100), AF488 donkey anti-rabbit (Dianova, 711-545-152, 1:100), AF647 donkey anti-mouse (Dianova, 715-605-151, 1:100), AF647 donkey anti-rabbit..



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    <t>a</t> <t>Exendin-4</t> reverses OGD-evoked mouse capillary constriction at pericytes; this is abolished by the AMPK blocker compound C (CC). b L-NAME does not affect OGD-evoked constriction, but abolishes GLP-1R-mediated dilation. c Atropine has no effect on the OGD-evoked constriction, but blocks the GLP-1R-mediated dilation in OGD. d Immunostaining of cardiac pericytes for Kir6.1 expression: cardiac tissue from wild type (WT), NG2-Cre +/− /Kir6.1 flx/flx (KO) mice and WT without Kir6.1 primary antibody. e Immunolabelled cardiac pericytes from four experimental groups of mice with LAD ligation: ischaemia/reperfusion only (IR), IR group pre-treated with CC (IR + CC), IR group pre-treated with Exendin-4 (IR+Ex4), and IR with both CC and Ex4 (IR + CC + Ex4). IB4 labelling extends all around the pericytes (white arrows). f Examples of Kir6.1 immunofluorescence intensity profiles measured with ImageJ, using <t>PDGFRβ</t> immunostaining to define cell surface. 10 line ROIs per cell (yellow lines) were drawn from the membrane inward in the PDGFRβ channel, transferred to the Kir6.1 channel, and fluorescence intensity averaged per pericyte. Distance from membrane to peak Kir6.1 immunofluorescence was averaged per heart ( n = 40, 43, 30, 31 pericytes in N = 5, 3, 3 and 3 hearts for IR, IR + CC, IR + Ex4 and IR + CC + Ex4). g Mean distance from membrane to peak Kir6.1 immunofluorescence intensity (also shown as red arrows in f ). One-way ANOVA with Šídák’s test. Data are mean ± SEM. Scale bar: 10 µm. Source data are provided as a file.
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    <t>a</t> <t>Exendin-4</t> reverses OGD-evoked mouse capillary constriction at pericytes; this is abolished by the AMPK blocker compound C (CC). b L-NAME does not affect OGD-evoked constriction, but abolishes GLP-1R-mediated dilation. c Atropine has no effect on the OGD-evoked constriction, but blocks the GLP-1R-mediated dilation in OGD. d Immunostaining of cardiac pericytes for Kir6.1 expression: cardiac tissue from wild type (WT), NG2-Cre +/− /Kir6.1 flx/flx (KO) mice and WT without Kir6.1 primary antibody. e Immunolabelled cardiac pericytes from four experimental groups of mice with LAD ligation: ischaemia/reperfusion only (IR), IR group pre-treated with CC (IR + CC), IR group pre-treated with Exendin-4 (IR+Ex4), and IR with both CC and Ex4 (IR + CC + Ex4). IB4 labelling extends all around the pericytes (white arrows). f Examples of Kir6.1 immunofluorescence intensity profiles measured with ImageJ, using <t>PDGFRβ</t> immunostaining to define cell surface. 10 line ROIs per cell (yellow lines) were drawn from the membrane inward in the PDGFRβ channel, transferred to the Kir6.1 channel, and fluorescence intensity averaged per pericyte. Distance from membrane to peak Kir6.1 immunofluorescence was averaged per heart ( n = 40, 43, 30, 31 pericytes in N = 5, 3, 3 and 3 hearts for IR, IR + CC, IR + Ex4 and IR + CC + Ex4). g Mean distance from membrane to peak Kir6.1 immunofluorescence intensity (also shown as red arrows in f ). One-way ANOVA with Šídák’s test. Data are mean ± SEM. Scale bar: 10 µm. Source data are provided as a file.
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    <t>a</t> <t>Exendin-4</t> reverses OGD-evoked mouse capillary constriction at pericytes; this is abolished by the AMPK blocker compound C (CC). b L-NAME does not affect OGD-evoked constriction, but abolishes GLP-1R-mediated dilation. c Atropine has no effect on the OGD-evoked constriction, but blocks the GLP-1R-mediated dilation in OGD. d Immunostaining of cardiac pericytes for Kir6.1 expression: cardiac tissue from wild type (WT), NG2-Cre +/− /Kir6.1 flx/flx (KO) mice and WT without Kir6.1 primary antibody. e Immunolabelled cardiac pericytes from four experimental groups of mice with LAD ligation: ischaemia/reperfusion only (IR), IR group pre-treated with CC (IR + CC), IR group pre-treated with Exendin-4 (IR+Ex4), and IR with both CC and Ex4 (IR + CC + Ex4). IB4 labelling extends all around the pericytes (white arrows). f Examples of Kir6.1 immunofluorescence intensity profiles measured with ImageJ, using <t>PDGFRβ</t> immunostaining to define cell surface. 10 line ROIs per cell (yellow lines) were drawn from the membrane inward in the PDGFRβ channel, transferred to the Kir6.1 channel, and fluorescence intensity averaged per pericyte. Distance from membrane to peak Kir6.1 immunofluorescence was averaged per heart ( n = 40, 43, 30, 31 pericytes in N = 5, 3, 3 and 3 hearts for IR, IR + CC, IR + Ex4 and IR + CC + Ex4). g Mean distance from membrane to peak Kir6.1 immunofluorescence intensity (also shown as red arrows in f ). One-way ANOVA with Šídák’s test. Data are mean ± SEM. Scale bar: 10 µm. Source data are provided as a file.
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    <t>a</t> <t>Exendin-4</t> reverses OGD-evoked mouse capillary constriction at pericytes; this is abolished by the AMPK blocker compound C (CC). b L-NAME does not affect OGD-evoked constriction, but abolishes GLP-1R-mediated dilation. c Atropine has no effect on the OGD-evoked constriction, but blocks the GLP-1R-mediated dilation in OGD. d Immunostaining of cardiac pericytes for Kir6.1 expression: cardiac tissue from wild type (WT), NG2-Cre +/− /Kir6.1 flx/flx (KO) mice and WT without Kir6.1 primary antibody. e Immunolabelled cardiac pericytes from four experimental groups of mice with LAD ligation: ischaemia/reperfusion only (IR), IR group pre-treated with CC (IR + CC), IR group pre-treated with Exendin-4 (IR+Ex4), and IR with both CC and Ex4 (IR + CC + Ex4). IB4 labelling extends all around the pericytes (white arrows). f Examples of Kir6.1 immunofluorescence intensity profiles measured with ImageJ, using <t>PDGFRβ</t> immunostaining to define cell surface. 10 line ROIs per cell (yellow lines) were drawn from the membrane inward in the PDGFRβ channel, transferred to the Kir6.1 channel, and fluorescence intensity averaged per pericyte. Distance from membrane to peak Kir6.1 immunofluorescence was averaged per heart ( n = 40, 43, 30, 31 pericytes in N = 5, 3, 3 and 3 hearts for IR, IR + CC, IR + Ex4 and IR + CC + Ex4). g Mean distance from membrane to peak Kir6.1 immunofluorescence intensity (also shown as red arrows in f ). One-way ANOVA with Šídák’s test. Data are mean ± SEM. Scale bar: 10 µm. Source data are provided as a file.
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    <t>a</t> <t>Exendin-4</t> reverses OGD-evoked mouse capillary constriction at pericytes; this is abolished by the AMPK blocker compound C (CC). b L-NAME does not affect OGD-evoked constriction, but abolishes GLP-1R-mediated dilation. c Atropine has no effect on the OGD-evoked constriction, but blocks the GLP-1R-mediated dilation in OGD. d Immunostaining of cardiac pericytes for Kir6.1 expression: cardiac tissue from wild type (WT), NG2-Cre +/− /Kir6.1 flx/flx (KO) mice and WT without Kir6.1 primary antibody. e Immunolabelled cardiac pericytes from four experimental groups of mice with LAD ligation: ischaemia/reperfusion only (IR), IR group pre-treated with CC (IR + CC), IR group pre-treated with Exendin-4 (IR+Ex4), and IR with both CC and Ex4 (IR + CC + Ex4). IB4 labelling extends all around the pericytes (white arrows). f Examples of Kir6.1 immunofluorescence intensity profiles measured with ImageJ, using <t>PDGFRβ</t> immunostaining to define cell surface. 10 line ROIs per cell (yellow lines) were drawn from the membrane inward in the PDGFRβ channel, transferred to the Kir6.1 channel, and fluorescence intensity averaged per pericyte. Distance from membrane to peak Kir6.1 immunofluorescence was averaged per heart ( n = 40, 43, 30, 31 pericytes in N = 5, 3, 3 and 3 hearts for IR, IR + CC, IR + Ex4 and IR + CC + Ex4). g Mean distance from membrane to peak Kir6.1 immunofluorescence intensity (also shown as red arrows in f ). One-way ANOVA with Šídák’s test. Data are mean ± SEM. Scale bar: 10 µm. Source data are provided as a file.
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    Image Search Results


    a Exendin-4 reverses OGD-evoked mouse capillary constriction at pericytes; this is abolished by the AMPK blocker compound C (CC). b L-NAME does not affect OGD-evoked constriction, but abolishes GLP-1R-mediated dilation. c Atropine has no effect on the OGD-evoked constriction, but blocks the GLP-1R-mediated dilation in OGD. d Immunostaining of cardiac pericytes for Kir6.1 expression: cardiac tissue from wild type (WT), NG2-Cre +/− /Kir6.1 flx/flx (KO) mice and WT without Kir6.1 primary antibody. e Immunolabelled cardiac pericytes from four experimental groups of mice with LAD ligation: ischaemia/reperfusion only (IR), IR group pre-treated with CC (IR + CC), IR group pre-treated with Exendin-4 (IR+Ex4), and IR with both CC and Ex4 (IR + CC + Ex4). IB4 labelling extends all around the pericytes (white arrows). f Examples of Kir6.1 immunofluorescence intensity profiles measured with ImageJ, using PDGFRβ immunostaining to define cell surface. 10 line ROIs per cell (yellow lines) were drawn from the membrane inward in the PDGFRβ channel, transferred to the Kir6.1 channel, and fluorescence intensity averaged per pericyte. Distance from membrane to peak Kir6.1 immunofluorescence was averaged per heart ( n = 40, 43, 30, 31 pericytes in N = 5, 3, 3 and 3 hearts for IR, IR + CC, IR + Ex4 and IR + CC + Ex4). g Mean distance from membrane to peak Kir6.1 immunofluorescence intensity (also shown as red arrows in f ). One-way ANOVA with Šídák’s test. Data are mean ± SEM. Scale bar: 10 µm. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: GLP-1 activates K ATP channels in coronary pericytes as the effector of brain-gut-heart signalling mediating cardioprotection

    doi: 10.1038/s41467-026-69555-1

    Figure Lengend Snippet: a Exendin-4 reverses OGD-evoked mouse capillary constriction at pericytes; this is abolished by the AMPK blocker compound C (CC). b L-NAME does not affect OGD-evoked constriction, but abolishes GLP-1R-mediated dilation. c Atropine has no effect on the OGD-evoked constriction, but blocks the GLP-1R-mediated dilation in OGD. d Immunostaining of cardiac pericytes for Kir6.1 expression: cardiac tissue from wild type (WT), NG2-Cre +/− /Kir6.1 flx/flx (KO) mice and WT without Kir6.1 primary antibody. e Immunolabelled cardiac pericytes from four experimental groups of mice with LAD ligation: ischaemia/reperfusion only (IR), IR group pre-treated with CC (IR + CC), IR group pre-treated with Exendin-4 (IR+Ex4), and IR with both CC and Ex4 (IR + CC + Ex4). IB4 labelling extends all around the pericytes (white arrows). f Examples of Kir6.1 immunofluorescence intensity profiles measured with ImageJ, using PDGFRβ immunostaining to define cell surface. 10 line ROIs per cell (yellow lines) were drawn from the membrane inward in the PDGFRβ channel, transferred to the Kir6.1 channel, and fluorescence intensity averaged per pericyte. Distance from membrane to peak Kir6.1 immunofluorescence was averaged per heart ( n = 40, 43, 30, 31 pericytes in N = 5, 3, 3 and 3 hearts for IR, IR + CC, IR + Ex4 and IR + CC + Ex4). g Mean distance from membrane to peak Kir6.1 immunofluorescence intensity (also shown as red arrows in f ). One-way ANOVA with Šídák’s test. Data are mean ± SEM. Scale bar: 10 µm. Source data are provided as a file.

    Article Snippet: To visualise GLP-1Rs in human pericytes, pericytes isolated from cardiac surgery waste tissue (cell isolation methods and characterisation as described previously ) were cultured to semi-confluency, fixed with 4% PFA for 20 min and labelled with the anti-human PDGFRβ antibody (Santa Cruz, sc-374573, 1:50) and Fluorescent Exendin-4 (Fluorescein-Trp25-Exendin-4, FLEX; Eurogentec, AS-63899, 100 nM).

    Techniques: Immunostaining, Expressing, Ligation, Immunofluorescence, Membrane, Fluorescence